The Wu Lab
The ability to rapidly identify functional proteins with post translation modifications (PTMs) is a crucial element in understanding cellular function. Protein PTMs have emerged in the post-genomic era critical features in regulating and diversifying protein biological activity, but identifying specific proteoforms with PTMs and understanding their function on individual proteins is currently limited by the lack of effective and accessible analytical methods. Therefore, there is a need to develop a high-throughput approach to functionally characterize intact proteins and their modified proteoforms at the systems level. The overall purpose of our research program is to develop and apply new top-down mass spectrometry (MS) and functional proteomics technologies in order to characterize intact proteins and their modified proteoforms.